Identification of an IL-7-Dependent Pre-T Committed Population in the Spleen

Abstract : Several extrathymic T cell progenitors have been described but their various contributions to the T cell lineage puzzle are unclear. In this study, we provide evidence for a splenic Lin−Thy1.2+ T cell-committed population, rare in B6 mice, abundant in TCRα−/−, CD3ε−/−, and nude mice, and absent in IL-7- and Rag-2-deficient mice. Neither B nor myeloid cells are generated in vivo and in vitro. The incidence of these pre-T cells is under the control of thymus and/or mature T cells, as revealed by graft experiments. Indeed, IL-7 consumption by mature T cells inhibits the growth of these pre-T cells. Moreover, the nude spleen contains an additional Lin−Thy1.2+CD25+ subset which is detected in B6 mice only after thymectomy. We establish that the full pre-T cell potential and proliferation capacity are only present in the c-kitlow fraction of progenitors. We also show that most CCR9+ progenitors are retained in the spleen of nude mice, but present in the blood of B6 mice. Thus, our data describe a new T cell lineage restricted subset that accumulates in the spleen before migration to the thymus.
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Soumis le : lundi 28 janvier 2019 - 13:57:33
Dernière modification le : jeudi 11 avril 2019 - 16:02:54

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L. Gautreau, L. Arcangeli, V. Pasqualetto, A.-M. Joret, C. Garcia-Cordier, et al.. Identification of an IL-7-Dependent Pre-T Committed Population in the Spleen. Journal of Immunology, Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists, 2007, 179 (5), pp.2925-2935. ⟨10.4049/jimmunol.179.5.2925⟩. ⟨hal-01996455⟩

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